THE SYNTHESIS OF GLUCOSE-6-PHOSPHATE AND 6-PHOSPHOGLUCONATE
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چکیده
منابع مشابه
The synthesis of glucose-6-phosphate and 6-phosphogluconate.
In studies of the enzymatic oxidation of 6-phosphogluconate, relatively large quantities of this substance in high purity were required. This led to a search for a convenient preparation of glucoseB-phosphate. Available methods for the synthesis of glucose-6-phosphate, reviewed by Lardy et al. (1, 2), are laborious and not well suited to the preparation of large quantities of this substance. A ...
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A simple microfluorometric method isdescribed for measu ring gIucose-6-phosphate dehyd rogenase (GPD) and 6-phosphogluconate dehydrogenase (PGD) activities in human erythrocytes. Only 50 ,J of whole blood is required. The cells are lysed with digitonin and the rate of NADPH generated in the enzymatic reactions is followed kinetically with a recording fluoromete. GPD activity is measured by subt...
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Resting potato tuber tissue possesses only faint activity of the two dehydrogenases of the oxi dative pentose phosphate cycle, glucose-6-phosphateand 6-phosphogluconate dehydrogenase. Slicing of the tissue, however, greatly enhances the action of both enzymes. The slicing-induced increase in activity is a consequence of intensified action of at least 5 glucose-6-phosphate dehydrogenase isozyme...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55918-4